In light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by dietinduced obesity dio 15, 16, atherosclerosisinduced by highcholesterol diet and in a. Overfeedinginduced obesity in zebrafish shows similar symptoms to those in. Backhed f, ding h, wang t, hooper lv, koh gy, nagy a, et al. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. Animal models of obesity are required to help us understand the signaling pathways underlying this condition.
Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear. One group of zebrafish was overfed with artemia 60 mg dry weightday fish, a living prey consisting of a relatively high amount of fat. The cape mountain specie prefers the mountainous areas of the eastern and western cape in southern africa. Hyperglycemia is the main feature for the diagnosis of this disease.
Research article open access dietinduced obesity in. Feeding hcd for 8 weeks resulted in exogenous obesity confirmed by higher body mass index bmi values of obese males 0. We investigated the effects of a simple carbohydraterich diet on the development of obesity related nafld and the impact of physical training on the metabolic abnormalities associated with this disorder. E2f8 promotes hepatic steatosis through fabp3 expression. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. Nutritional factors play important roles in the etiology of obesity, type 2 diabetes mellitus and their complications through genotype x environment interactions. Highfat dietinduced obesity triggers alveolar bone loss and. Epigenetic regulation of adipogenesis by phf2 histone. Nov 29, 2012 carbohydratefree diet for 2 or 4 weeks following protocols. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Mar 21, 2017 different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14.
Nov 23, 2015 obesity is a multifactorial health problem resulting from genetic, environmental and behavioral factors. The zone diet has been consistently demonstrated in controlled clinical. Can a high fat paleo diet cause obesity and diabetes. We optimized protocols for generating dietinduced obese dio zebrafish model by feeding high cholesterol diet hcd. Fatty diet is a significant factor causing obesity. Barry sears, to reduce the levels of dietinduced inflammation.
The first dietinduced obese zebrafish model dio was reported in 2010 through. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or. In this paper, hyperglycemic zebrafish, when immersed in a 111 mm glucose solution for 14 days, developed increased glycation of proteins. Different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14. Dietinduced obesity in male c57bl6 mice decreases fertility. Although the zebrafish is a model that has become popular in recent decades for basic biomedical research due to the multiple advantages highlighted in several articles 40,41, it has not been used extensively for studies related to obesity and diet. These diets were based the ain93g diet and beef tallow was added to provide a highfat content. Zeigler zebrafish diet, 1lb bag during this covid19 pandemic, pentair global operations and supply teams are working diligently to help ensure our valued customers are getting the best possible service and delivery during this time. Consequently, zebrafish might serve as a good animal model for. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity. Diabetes mellitus dm affects over 10% of the worlds population.
Dietinduced dysbiosis of the intestinal microbiota and. Hf and hfc dietsinduced hepatic steatosis in zebrafish larvae. We have characterised molecular adaptation to high fat diet hfd feeding in inbred mouse strains widely used in genetic and physiological studies. Dec 19, 2011 effects of yuzu citrus junos siebold ex tanaka peel on the diet induced obesity in a zebrafish model journal of functional foods, vol.
Macrophages are ubiquitously present innate immune cells in humans and animals belonging to both invertebrates and vertebrates. Dec 22, 2017 an increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Fatrich diets not only induce obesity in humans but also make animals obese. All materials tested exhibited anti obesity effects. Anti obesity mechanisms of pm in zebrafish and mice models of obesity. Turnbaugh pj, ley re, mahowald ma, magrini v, mardis er, gordon ji. Pdf dietinduced obesity in zebrafish shares common. Less than 5% of chemicals were eluted from gluten granules. However, it is as yet unclear whether highfat diet hfd induced obesity in childhood and adolescence determines agerelated changes in jaw bone health. In this study, we found that pacific dulse, palmaria mollis pm, showed anti obesity effects in diet induced obesity models. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets.
In this study, it was purposed to examine histological effects of fatty diet induced obesity on liver from female obese rats. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 %. Reactive oxygen species and oxidative stress in obesity. Researchers now describe the first genetic model of obesity in a fish. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. The zone diet is a nutritional program developed by dr. Dietinduced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity article pdf available in bmc physiology 101. Body fat accumulation in zebrafish is induced by a diet. This was an extremely high fat diet for mice, enough to render them obese and or diabetic in 4 weeks. The zebrafish danio rerio is an established model organism for the study of various metabolic diseases. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of. Thus, zebrafish are a useful model of human dietinduced obesity, which. A particularly interesting aspect of obesity is the differences observed in response to the.
The pathogenesis of nonalcoholic fatty liver disease nafld is not fully understood, and experimental models are an alternative to study this issue. Highfat dietinduced obesity triggers alveolar bone loss. Short chain fatty acids prevent highfatdietinduced. There were some differences between zebrafish and mice in how pm exerts antiobesity mechanisms. E2f8 promotes hepatic steatosis through fabp3 expression in. Short chain fatty acids prevent highfat diet induced obesity in mice by regulating g proteincoupled receptors and gut microbiota. A novel protocol for the oral administration of test. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 % and 11 %, respectively. Novel antiobesity properties of palmaria mollis in zebrafish. The aim of this study was to generate an obese adult zebrafish. A novel wistar rat model of obesityrelated nonalcoholic. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. These data show that hyperglycemia can be induced and maintained in zebrafish. In four experiments, longevans rats ate ad libitum a synthetic semipurified diet containing 20 g hf or 4 g lowfat lf of fat100 g of diet or a nonpurified diet.
All materials tested exhibited antiobesity effects. Highfat diet impairs cognitive function of zebrafish. We developed a rat model of diet induced obesity that will be a powerful tool for assessment of this issue. Short chain fatty acids prevent highfatdietinduced obesity in mice by regulating g proteincoupled receptors and gut microbiota. The aim of this study is to examine the agerelated influence of hfdinduced obesity on mandibular bone architecture and. Nutrigenomics of high fat diet induced obesity in mice.
Diet induced obese dio mouse models c57bl6ntac male mice. Typically mice, rats, dogs, or nonhuman primates are used in these models. Basic nutritional investigation ingested cocoa can prevent. Obesity is a multifactorial health problem resulting from genetic, environmental and behavioral factors. Sucrose nonfermentingrelated kinase snrk is a member of the ampkrelated kinase family, and its physiological role in adipose energy homeostasis and inflammation remains unknown. In this study, we examined the in vivo role of phf2 in mice. Zebrafish as an experimental model for the study of obesity. May 20, 2015 in light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by diet induced obesity dio 15, 16, atherosclerosis induced by highcholesterol diet and in a. Dietinduced obesity in zebrafish shares common pathophysiological pathways. Fatrich diets not only induce obesity in humans but also make.
An obesityassociated gut microbiome with increased capacity for energy harvest. The dietinduced obesity model closely mimics the increasingly availability of the highfathighdensity foods in modern society over the past two decades, which are main contributors to the obesity trend in human. Backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of dietinduced obesity.
We generated phf2 floxed mice, systemic phf2 null mice by crossing phf2 floxed mice with cmvcre transgenic mice, and tamoxifen. Moreover, a useful zebrafish model of diet induced obesity dio has been created and was found to be highly consistent with the obesity observed in humans and in rodent models of dio oka et al. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Compositions of real and mimetic cocoa are listed in table 2. Adult zebrafish have also been used as an obesity model. Our results suggest that a diet rich in fat, instead of protein or carbohydrate, induced body fat accumulation in zebrafish with mechanisms that might be similar to those in mammals. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear because of reported. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. The gut microbiota as an environmental factor that regulates fat storage. The aim of this study was to examine the antiobesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Childhood obesity and the zebrafish as a model for the. Dietinduced obesity in c57bl6j mice causes increased. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with diet induced obesity dio were evaluated.
We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks. Since rats and mice show a similar relationship, they are considered an appropriate model. We determined the antiobesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of. Sucrose nonfermentingrelated kinase regulates both adipose. These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of phagocytosis of. Effects of yuzu citrus junos siebold ex tanaka peel on. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical. Jun 19, 2007 everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity. Dietinduced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.
Jun 24, 2012 backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. High fat plus high cholesterol diet lead to hepatic. There were some differences between zebrafish and mice in how pm exerts anti obesity mechanisms. Most of those studies compared lean and obese animals and were based on excessive overfeeding of adult zebrafish with their natural diet in. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Overfed diet induced obesity dio zebrafish exhibit increased body weight gain, hypertriglyceridemia, hepatic steatosis 23,24, and visceral adiposity 25,26. Controlled highfat diet induces an obese syndrome in rats. The first diet induced obese dio zebrafish model was reported in 2010 oka et al.
These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Rat models of dietinduced obesity and high fatlow dose. Future studies will enable us to understand the composite nature of mitochondrial flash activity and will encourage the generation of new indicators to better measure these phenomena. In this study, we used a dietinduced obesity approach in zebrafish danio.
Everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. Adult zebrafish were allocated to four diet groups and over 6 weeks. The effect of diet and exercise on intestinal integrity and microbial diversity in mice. Highfat diet impairs cognitive function of zebrafish scientific reports. The diet contained 72% fat corn oil and lard, 28% protein, and 1% carbohydrate as energy content 17. In this study, we found that pacific dulse, palmaria mollis pm, showed antiobesity effects in dietinduced obesity models. Although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for diet induced obesity dio, similar to that observed in mammals. Sucrose nonfermentingrelated kinase regulates both. An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Proofofconcept using pharmacokinetics and pharmacological evaluation of carbamazepine. We previously reported that snrk is ubiquitously and abundantly expressed in both white adipose tissue wat and brown adipose tissue bat, but snrk expression diminishes. Related publications genetic determinants of energy expenditure and insulin resistance in dietinduced obesity in mice. Adult danio rerio zebrafish represents a powerful model to study both metabolic diseases and bone metabolism.
Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical the zebrafish is an important and widely used vertebrate model organism in scientific. The purpose of this study was to establish a zebrafish model of dietinduced obesity dio. Hf or hfc diets exposure results in steatosis and hepatitis in adult fish and rodents models 25, 35. Effects of glucose treatment up to 14 days on blood glucose levels. Diet induced obese dio mouse models c57bl6ntac male. Antiobesity mechanisms of pm in zebrafish and mice models of obesity.
Metabolic and bone effects of highfat diet in adult zebrafish. Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type ii diabetes, hypertension and cancer. A novel protocol using gluten as a carrier material was developed to administer chemicals to adult zebrafish, per os p. Lipidomic profiling of liver tissue from obesityprone and. Research article a novel wistar rat model of obesity. Yuzu peel significantly suppressed the rise in plasma triacylglycerol tg and liver lipid accumulation. This model has lead to many discoveries of the important signalings in obesity, such as akt and mtor. The organs that show signs of adiposity, such as fat accumulation in the liver and visceral adipose tissues, are similar to those found in humans. We investigated the effects of a simple carbohydraterich diet on the development of obesityrelated nafld and the impact of physical training on the metabolic abnormalities associated with this disorder. However, it is as yet unclear whether highfat diet hfdinduced obesity in childhood and adolescence determines agerelated changes in jaw bone health.
The gi tract functions as a major immunological organ as it must maintain tolerance to commensal and dietary antigens while remaining responsive to pathogenic stimuli. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of obesity. We carried out physiological tests, plasma lipid assays, obesity. Dietinduced adipocyte number increase in adult rats. High fat plus high cholesterol diet lead to hepatic steatosis. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with dietinduced obesity dio were evaluated. We carried out physiological tests, plasma lipid assays, obesity measures, liver. The jackson laboratory maintains an inventory of diet induced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them.
Rnaseq based transcriptome analysis of the antiobesity effect of. International database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data. Gte decreased visceral adipose tissue vat volume induced by highfat diet in zebrafish larvae. The burchells zebra prefer open woodland, scrub and grassland. Antiobesity effects of boiled tuna extract in mice with. Diet induced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. To evaluate the capacity of gluten to retain chemicals, we prepared gluten granules containing eight types of chemicals with different log p ow values and immersed them in water. Obesity is a multifactorial disorder influenced by genetic and environmental factors. Rats were examined for effects of cocoa ingestion with highfat diets. The present study was performed to investigate the molecular mechanism of 6gingerol on adipocytemediated systemic inflammation in vitro and in highfat dietinduced obese zebra fish. An eaters guide to a healthy microbiome human food project. Novel antiobesity properties of palmaria mollis in. The gastrointestinal gi microbiota is the collection of microbes which reside in the gi tract and represents the largest source of nonself antigens in the human body.
Dietinduced obesity in c57bl6j mice causes increased renal. Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. International database resource for the laboratory mouse, providing integrated. They are very dependent on water and you will seldom see them more than 12 km from it. We previously reported that snrk is ubiquitously and abundantly expressed in both white adipose tissue wat and brown adipose tissue bat, but snrk expression diminishes in adipose tissue in obesity. Zebrafish is emerging as an important model for studying obesity and related. Effects of yuzu citrus junos siebold ex tanaka peel on the. Propensity to highfat dietinduced obesity in rats is. It is known that obesity is associated with many chronic disorders such as nonalcoholic fatty liver disease nafld. Phf2 is a jmjc family histone demethylase that removes the methyl group from h3k9me2 and works as a coactivator for several metabolismrelated transcription factors.
Oct 21, 2010 obesity is a multifactorial disorder influenced by genetic and environmental factors. The relationship between highfat food consumption and obesity is wellestablished. The aim of this study is to examine the agerelated influence of hfd induced obesity on mandibular bone architecture and the structure of the periodontium in. The purpose of this study was to establish a zebrafish model of diet induced obesity dio. Persistent impaired glucose metabolism in a zebrafish. To determine whether this also occurs in larvae, zebrafish fed with hf diet and hfc diet were stained with oil red o.
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